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Extended sonication should be avoided as it may degrade the peptide
16 weeks 1 x 10 mL bottle Covers 4-5 vials per bottle 26-32 weeks 2 x 10 mL bottles Margin for priming losses 68 weeks 4 x 10 mL bottles Long-cycle margin Round up for priming losses, dropped syringes, damaged swabs, and any protocol adjustments
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These motions are consistent with the loss of key interactions mediated by Tyr 37 of amylin peptides and the poor resolution of the RAMP3 ECD in the 3D EM map reconstructions
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